ABSTRACT
Background and Aims: Chromosome abnormalities are a major cause of miscarriage and neonatal mortality. The present study aimed to determine the association of fetal and parents chromosomal abnormalities with congenital anomalies
Methods: A cross-sectional study was performed in a tertiary referral center [Afzalipour Hospital] over 16 months period [2011-2012]. The study groups consisted of 77 fetuses over 14 weeks and their parents. Fetuses had apparent anomaly after abortion or birth, or showed a defect organs in targeted sonographic examination. DNA extractions were from fetus tissues for investigation of chromosome abnormalities using [multiplex ligation-dependent probe amplification] MLPA. Cytogenetic analysis for parents was performed with G-banding technique. Eventually data were analyzed by statistical software SPSS using t-test, chisquare and logistic regression
Results: Karyotyping of fetus was 46xx in 27 [35.9%] and 46xy in 25 [32.1%] cases. Twenty-five fetuses had chromosomal abnormalities. The common chromosomal abnormalities were multiple deletion and duplication on different chromosomes in 4 [6.5%] and Down syndrome in 3 [3.9%] of them. This study showed a statistically significant association between the extremity anomalies [P=0.0070], oligohydroamnious [P=0.0050], ascitis [P=0.0001], increased nuchal translucency [P=0.0001], esophageal atresia [P=0.0070], duodenal atresia [P=0.0001], polycystic kidney [P=0.0070], echogenic bowel [P=0.0001], plural effusion [P=0.0001] and cardiomegaly [P=0.0010]. There were no statistically significant association between the chromosomal abnormalities in fetus and parents [P=0.5700]
Conclusion: In our study, there was no association between the chromosomal abnormalities in fetus and parents. It can be concluded that many chromosomal defects occur during the formation of sperm or ovum. Detection of major congenital malformations should draw attention to the possibility of a chromosomal disorder in fetus. Therefore, MLPA is a low cost method for detecting a wide range of the most common chromosomal disorders in a short time
ABSTRACT
Over the past decade the US army has widely studied new technologies for stopping sever hemorrhages and has introduced an effective Zeolite based hemostatic agent. On the other hand, Mortazavi and his colleagues previously reported the bio-stimulatory effects of the topical application of radioactive lantern mantle powder on wound healing. Their subsequent studies showed significant changes in some histological parameters concerning healing. In this light, here the bio-stimulatory effect of burned radioactive lantern mantles powder as well as two minerals; bentonite and zeolite arepresented. This experimental study was conducted in the center for radiological studies, Shiraz university of Medical Sciences in 2008. Fifty male Wistar rats were divided randomly into 5 groups of 10 animals each. Following anesthesia, animals' tails were cut at a thickness of 5 mm by using a surgical scissor. No intervention was made on the animals of the 1[st] group. The 2[nd] group to 4[th] group received topical non-radioactive lantern mantle powder, radioactive lantern mantle powder, Bentonite mineral or a mixture of Bentonite-Zeoliteat minerals respectively. After treatment with above mentioned agents, the volume of blood loss was measured using a scaled test-tube. The bleeding time and clotting time were also measured using a chronometer. SPSS software was used for statistical analysis. ANOVA was used for comparing the means of each parameter in the 5 groups. The volume of blood loss, bleeding and clotting times in control animals were 4.39 +/- 1.92 cc, 112.10 +/- 39.60 sec and 94.9 +/- 54.26 sec, respectively. In the 5[th] group in which the animals were treated with a mixture of Bentonite-Zeoliteat minerals, the volume of blood loss, bleeding and clotting times were 1.31 +/- 0.60 cc, 34.50 +/- 4.65 sec and 24.2 +/- 4.61 sec, respectively. This is the 1[st] investigation that studied the alterations of bleeding and clotting times following the use of lantern mantle powder as well as bentonite or the mixture of bentonite-zeolite minerals. The results obtained in this study clearly show the significant effect of the volume of blood loss as well as the bleeding time following the topical use of the mixture of bentonite-zeolite minerals. The radioactive and non-radioactive lantern mantle and the bentonite mineral alone had considerable effects but overally the magnitude of the effect was less than that of the mixture of bentonite-zeolite minerals